The size exclusion cutoff is in the range of Mr 700 000 making Capto Core 700 an excellent tool for purification challenges for large biomolecules, such as IgM or influenza viruses. Previously, extracellular vesicles (EVs) were thought to serve. Here we show purification examples with Capto Core 700 on two examples of large biomolecules, IgM, and influenza virus.Ĭapto Core 700 is built with core-bead technology that allows double functionality – size exclusion and binding separation in one bead. purification, separation, exosome markers, Capto Core 700, Capto Heparin. (B) Capto Core 700 this novel resin combines size exclusion chromatography (SEC) and mixed-mode anion exchange characteristics. After passing the Capto Core 700 column, the VLPs were disassembled into L1 protein monomers by disruption of disulfide bonds between the L1 proteins using dithiothreitol (DTT). The benefits of this technology include high loading, high flow rates with short residence times, and robust performance in a wide window of operations, securing high productivity and straightforward process design. The filtrate was run in flow-through mode on Capto Core 700 medium for removal of impurities. The core bead technology enables double functionality for Capto Core 700 – size exclusion and binding separation in one bead. Novel core bead technology and multimodal ligand give Capto Core 400 dual functionality, size exclusion and binding chromatography, in one chromatography resin. Capto™ Core 700 is a chromatography medium (resin) from GE Healthcare Life Sciences designed with core bead technology. Each bead has a ligand-activated core and an outer layer without ligands. PreDictor 96-well plates were filled with 10 L of Capto Core 700 for the binding study. The product is based on the core bead concept. The 3 steps purification protocol uses a recently developed multimodal size-exclusion chromatography medium (Capto Core 700) in combination with detergent extraction and size-exclusion polishing to reach a 89 VLP purity with a 19 yield. Capto Core 700 The binding capacity of Capto Core 700 for host cell protein (HCP) from MDCK cell lysate was evaluated in buffers containing sodium phosphate and Tris, 1501000 mM NaCl, pH 6.58.0. Advantages of using gel filtration include the high purity and the high recoveries that can be achieved, while a drawback is the relatively low productivity. Capto Core 700 chromatography medium is aimed at intermediate purification and polishing of viruses and other large biomolecules in flow- through mode. When large biomolecules such as IgM or influenza virus are purified, group separation with gel filtration is the most commonly used technique.
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